Specificity in DNA recognition by phage integrases
| Autor: | Alice del-Campillo-Campbell, Michael L. Ginsberg, Allan Campbell |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_classification
Genetics DNA Bacterial Binding Sites Base Sequence Integrases Oligonucleotide Virus Integration Oligonucleotides Genetic Variation General Medicine Biology Site specificity Bacteriophage lambda Amino acid Substrate Specificity chemistry Polymorphism (computer science) Bacteriophages Selection Genetic Dna recognition Recombination Functional polymorphism |
| Zdroj: | Gene. 300(1-2) |
| ISSN: | 0378-1119 |
| Popis: | The lambda-related (lambdoid) coliphages are related to one another by frequent natural recombination and maintain a high level of functional polymorphism for several activities of the phages. Arguments are presented that the polymorphism of the integration module results from selection (presumably frequency-dependent) for new (not improved) specificities of site recognition. Analysis of phages lambda and HK022 by Weisberg and collaborators previously showed that changes in five noncontiguous amino acids could switch site recognition specificity. Phage 21 and defective element e14, which integrate at the same site, differ in recognition specificity for both core and arm sites. In vitro assays of e14 and 21 insertion and excision confirm this conclusion. Inhibition by ds arm site oligonucleotides defines the sequence specificity more precisely. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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