DNA microarray analysis of the correlation between gene expression patterns and acquired resistance to 5-FU/cisplatin in gastric cancer
| Autor: | In-Hoo Kim, Eugene Kim, Jin Soo Lee, Il Ju Choi, Hark Kyun Kim, Jae-Moon Bae, Ju Han Kim, Il-Jin Kim, Insook Park, Jong Ho Chun, Hio Chung Kang, Hee Sung Kim, Jae-Gahb Park, Jae-Hyun Park |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Antimetabolites Antineoplastic Microarray Biopsy Biophysics Down-Regulation Drug resistance Biology Biochemistry Stomach Neoplasms DNA Microarray Analysis Gene expression medicine Cluster Analysis Humans Molecular Biology Aged Oligonucleotide Array Sequence Analysis Regulation of gene expression Cisplatin medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Cancer Endoscopy Cell Biology Middle Aged medicine.disease Molecular biology Up-Regulation Gene Expression Regulation Neoplastic Drug Resistance Neoplasm Disease Progression Cancer research Female Fluorouracil medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 316:781-789 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2004.02.109 |
| Popis: | The mechanisms of intrinsic and/or acquired anti-cancer drug resistance have been described in in vitro resistance models, but the clinical relevance has remained undefined. We undertook a prospective study to identify correlations between gene expression and clinical resistance to 5-FU/cisplatin. We compared expression profiles from gastric cancer endoscopic biopsy specimens obtained at a chemosensitive state (partial remission after 5-FU/cisplatin) with those obtained at a refractory state (disease progression), using Affymetrix oligonucleotide microarray technology (U133A). Using 119 discriminating probes and a cross-validation approach, we were able to correctly identify the chemo-responsiveness of 7 pairs of training samples and 1 independent test pair. These exploratory data demonstrate that the gene expression profiles differ between chemosensitive and refractory state gastric cancer biopsy samples. |
| Databáze: | OpenAIRE |
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