Effect of interferon-γ on purine catabolic and salvage enzyme activities in rats
Autor: | Yuji Moriwaki, Zenta Tsutsumi, Jun-ichi Yamakita, Kazuya Higashino, Tetsuya Yamamoto, Sumio Takahashi |
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Rok vydání: | 1999 |
Předmět: |
Male
Purine Hypoxanthine Phosphoribosyltransferase Xanthine Oxidase Adenosine Deaminase Xanthine Dehydrogenase Adenine Phosphoribosyltransferase Biophysics Adenine phosphoribosyltransferase Purine nucleoside phosphorylase Biochemistry Interferon-gamma chemistry.chemical_compound Adenosine deaminase Animals RNA Messenger Rats Wistar Enzyme inducer Molecular Biology biology Molecular biology Enzyme assay Rats Liver Purine-Nucleoside Phosphorylase chemistry Xanthine dehydrogenase Hypoxanthine-guanine phosphoribosyltransferase biology.protein |
Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 1427:385-391 |
ISSN: | 0304-4165 |
DOI: | 10.1016/s0304-4165(99)00038-0 |
Popis: | To determine whether interferon-gamma affects rat purine catabolic and salvage enzyme activities, rats were injected with interferon-gamma (600000 U/kg, i.p.) and, similarly to a vehicle-injected control group, killed before or after injection at 6, 12, and 24 h. Organ homogenates were prepared and enzymatic reactions with substrates were carried out, after which the products were measured either chromatographically or spectrophotometrically. Western and Northern blotting also were performed. In contrast to the vehicle-injected rats, interferon-gamma-injected rats showed a significant rise in xanthine oxidoreductase activity in the liver, while enzyme activity was unchanged in the spleen, kidney, and lung. Western analysis of hepatic xanthine oxidoreductase showed an increased concentration of this protein 12 and 24 h after interferon-gamma injection. Northern analysis disclosed an enhanced mRNA expression coding for this enzyme, peaking 12 h after injection. Contrastingly, the activities of adenosine deaminase, purine nucleoside phosphorylase, hypoxanthine guanine phosphoribosyltransferase, and adenine phosphoribosyltransferase were not affected by interferon-gamma in any organ tested. While interferon-gamma causes an increased hepatic biosynthesis of xanthine oxidoreductase, the physiologic role of this enzyme induction remains undetermined. |
Databáze: | OpenAIRE |
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