Adaptation of Saccharomyces cerevisiae to growth on cholesterol: Selection of mutants defective in the formation of lanosterol
| Autor: | Frederick R. Taylor, L. W. Parks |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Period (gene)
Saccharomyces cerevisiae Mutant Biophysics Biology Biochemistry Lanosterol chemistry.chemical_compound Ergosterol polycyclic compounds Molecular Biology Cholesterol Cell Biology Hydrogen-Ion Concentration Lipid Metabolism biology.organism_classification Adaptation Physiological Sterol chemistry Mutation lipids (amino acids peptides and proteins) Adaptation |
| Zdroj: | Biochemical and Biophysical Research Communications. 95:1437-1445 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(80)80058-1 |
| Popis: | Summary The ability of cholesterol to support the growth of a heme-deficient mutant of Saccharomyces cerevisiae has been examined. Growth was slow for an extended period of time until an “adaptation” occurred resulting in faster growth. This adaptation has proven to be the selection of spontaneous mutants in steps of the sterol biosynthetic pathway just prior to the formation of lanosterol. This observation is consistent with the detrimental effects of lanosterol on membrane properties. Our “adapted” strains grow over a narrower range of pH on cholesterol as compared to ergosterol. |
| Databáze: | OpenAIRE |
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