Chondrocyte Co-cultures with the Stromal Vascular Fraction of Adipose Tissue in Polyhydroxybutyrate/Poly-(hydroxybutyrate-co-hydroxyhexanoate) Scaffolds: Evaluation of Cartilage Repair in Rabbit

Autor: Xue-Qin Wei, Xin-Bo Wang, Wenting Pan, Duan Ni, Tianfeng Du, Na Li, Kai Ba
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Cartilage
Articular
Polyesters
0206 medical engineering
Biomedical Engineering
chondrocytes
Adipose tissue
Hydroxybutyrates
lcsh:Medicine
02 engineering and technology
Knee Injuries
Chondrocyte
Polyhydroxybutyrate
In vivo
Cell Movement
Absorbable Implants
medicine
Cell Adhesion
Animals
Regeneration
Caproates
Cells
Cultured
Cell Proliferation
Transplantation
Wound Healing
3-Hydroxybutyric Acid
Tissue Engineering
Tissue Scaffolds
Chemistry
Cartilage
Regeneration (biology)
lcsh:R
Cell Biology
Original Articles
Stromal vascular fraction
021001 nanoscience & nanotechnology
020601 biomedical engineering
co-culture
stromal vascular fraction
Coculture Techniques
Cell biology
medicine.anatomical_structure
Adipose Tissue
Microscopy
Electron
Scanning
cartilage repair
Rabbits
Stem cell
Stromal Cells
0210 nano-technology
Zdroj: Cell Transplantation, Vol 28 (2019)
Cell Transplantation
ISSN: 1555-3892
0963-6897
Popis: Chondral defects are challenging to repair because of the poor self-healing capacity of articular cartilage. The aim of this study was to compare and investigate the cartilage regeneration of stromal vascular fraction (SVF) cells and adipose-derived stem cells (ASCs) co-cultured with chondrocytes seeding on scaffolds composed of polyhydroxybutyrate (PHB)/poly-(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx). In this study, the cellular morphologies and proliferation capabilities on scaffolds were evaluated. Next, scaffolds with 1:1 co-culture of ASCs/SVF and chondrocytes were implanted into the full-thickness cartilage defects in rabbit knee for 10 weeks. Cells seeded on the scaffolds showed better adhesion, migration, and proliferation in vitro. Importantly, implantation with scaffolds with SVF and chondrocytes revealed more desirable in vivo healing outcomes. Our results illustrate a one-step surgical procedure for the regeneration of focal cartilage defects using a mixture of SVF from adipose tissue and uncultured chondrocytes.
Databáze: OpenAIRE
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