Murine response to DNA encoding herpes simplex virus type-1 glycoprotein D targeted to the liver
| Autor: | Nancy J. Bigley, Pamela S. Fink, Henry C. Chiou, Barbara E. Hull, James V. Rogers |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Asialoglycoproteins
Herpesvirus 1 Human medicine.disease_cause Herpesviridae Virus law.invention Mice chemistry.chemical_compound Plasmid Viral Envelope Proteins law Alphaherpesvirinae Gene expression Vaccines DNA medicine Animals Polylysine Mice Inbred BALB C General Veterinary General Immunology and Microbiology biology Public Health Environmental and Occupational Health Viral Vaccines Orosomucoid biology.organism_classification Immunohistochemistry Virology Infectious Diseases Herpes simplex virus Liver chemistry Recombinant DNA Molecular Medicine Female Immunization DNA |
| Zdroj: | Vaccine. 18:1522-1530 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(99)00438-7 |
| Popis: | Plasmid DNA encoding herpes simplex virus type-1 glycoprotein D (gD-1) was complexed with asialoorosomucoid conjugated to poly-L-lysine. Following its intravenous injection into BALB/c mice, this complex was targeted to the liver. Liver cells expressing gD-1 were detected immunohistochemically through day 6 post-immunization, while gD-1 DNA was detectable through 14 days post-immunization. Decline of gD-1 expression and detectable gD-1 DNA in the liver correlated with influx of T cells, predominantly CD4(+). The ASOR-poly-L-lysine DNA carrier system promotes hepatic expression of gD-1 and may be useful in vaccination against herpes simplex virus type-1. |
| Databáze: | OpenAIRE |
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