High temperature inhibits the accumulation of storage materials by inducing alternative splicing of OsbZIP58 during filling stage in rice
| Autor: | Chunshou Li, Wang Liangchao, Xiaofang Li, Ying Zhu, Hua Zhang, Jiping Gao, Zhengge Zhu, Heng Xu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Starch Plant Science 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Grain quality Storage protein Gene Transcription factor Plant Proteins chemistry.chemical_classification Alternative splicing Temperature food and beverages Oryza Plants Genetically Modified Endosperm Cell biology Storage material Alternative Splicing 030104 developmental biology chemistry Mutation Seeds alpha-Amylases Function (biology) Transcription Factors 010606 plant biology & botany |
| Zdroj: | Plant, Cell & Environment. 43:1879-1896 |
| ISSN: | 1365-3040 0140-7791 |
| DOI: | 10.1111/pce.13779 |
| Popis: | High temperature (HT) has an adverse effect on rice grain filling by inhibiting the accumulation of storage materials. However, the regulatory mechanism of this inhibition remains unknown. Here, we report that Opaque2 like transcription factor OsbZIP58 is a key factor regulating storage material accumulation under HT. The OsbZIP58 gene promotes expression of many seed storage protein genes and starch synthesis genes while inhibits expression of some starch hydrolyzing α-amylase genes under HT. The loss of OsbZIP58 function leads to floury and shrunken endosperms and dramatically reduced storage materials in the seeds under HT. HT is found to affect alternative splicing of OsbZIP58, promoting the formation of the truncated OsbZIP58β protein form over the full-length OsbZIP58α protein form. The OsbZIP58β form has a lower transcriptional activity than the OsbZIP58α form, especially under HT condition. Interestingly, rice varieties with less heat sensitivity have reduced alternative splicing of OsbZIP58. Therefore, OsbZIP58 is a crucial gene in regulating storage material accumulation under HT and lower alternative splicing of OsbZIP58 may contribute to heat tolerance during grain filling. |
| Databáze: | OpenAIRE |
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