Cigarette smoking affects microRNAs and inflammatory biomarkers in healthy individuals and an association to single nucleotide polymorphisms is indicated
| Autor: | Lars Erik Rutqvist, Sture Löfgren, Nongnit Laytragoon-Lewin, Shariel Sayardoust, Bengt-Åke Andersson |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Health Toxicology and Mutagenesis Clinical Biochemistry Single-nucleotide polymorphism Inflammation 030204 cardiovascular system & hematology Biochemistry Polymorphism Single Nucleotide Cigarette Smoking Epigenesis Genetic Pathogenesis 03 medical and health sciences Interferon-gamma 0302 clinical medicine Immune system Cigarette smoking microRNA Leukocytes Medicine Humans Epigenetics Chemokine CCL2 Aged Smokers business.industry Middle Aged Immunity Innate MicroRNAs C-Reactive Protein 030220 oncology & carcinogenesis Healthy individuals Immunology Female medicine.symptom business Biomarkers |
| Zdroj: | Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals. 24(2) |
| ISSN: | 1366-5804 |
| Popis: | Cigarette smoke induces inflammation and remodels immune response. Genetic and epigenetic alterations might be involved in the pathogenesis of smoking related diseases. In this study, we investigated the effect of smoking on systemic inflammation biomarkers and epigenetic changes at microRNA (miRNA) expression level. We also examined if the levels of inflammatory biomarkers were associated with selected single nucleotide polymorphisms (SNPs).From 39 smokers and 101 non-smokers, levels of total white blood cells (WBCs) and its subpopulations, plasma cytokines/chemokines/proteins and miRNAs were analysed. For three biomarkers, C-reactive protein (CRP), MCP-1 and IFN-γ that were affected by smoking, the influence of SNPs was analyzed.Elevated levels of total WBCs, neutrophils, monocytes, lymphocytes, CRP, MCP-1, IFN-γ and lower levels of miR-21 were detected in smokers. The elevated levels of IFN-γ in smokers was only statistically significantly associated with rs2069705 AG/GG SNP-genotype.A lower level of oncomir miRNA-21 and a higher level of immune modelling cytokine IFN-γ detected in smokers could be a protective immune response to cigarette smoke. The higher level of IFN-γ in smokers with a specific SNP genotype also suggests that a genetic interaction with smoking might predict the pathobiology of smoking related disease. |
| Databáze: | OpenAIRE |
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