Two novel conjugative plasmids from a single strain of Sulfolobus
| Autor: | Wolfram Zillig, H. J. G. van de Werken, Kenneth M. Stedman, J. van der Oost, Gaël Erauso |
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| Přispěvatelé: | Immunology |
| Rok vydání: | 2006 |
| Předmět: |
Transcription
Genetic mobile elements Operon Genetic Vectors Molecular Sequence Data ved/biology.organism_classification_rank.species Gene Dosage Biology Microbiology Genome Sulfolobus Open Reading Frames trag-like proteins Plasmid Genome Archaeal Microbiologie Host chromosome complete nucleotide-sequence thermophilic archaeon sulfolobus VLAG Recombination Genetic shuttle vectors Genetics Base Sequence ved/biology Bacterial conjugation Sulfolobus solfataricus hyperthermophilic archaeon biology.organism_classification genetic elements bacterial conjugation DNA Transposable Elements crenarchaeon sulfolobus transcription regulation Low copy number Plasmids |
| Zdroj: | Microbiology, 152(7), 1951-1968. Microbiology Society Microbiology 152 (2006) 7 Microbiology, 152(7), 1951-1968 |
| ISSN: | 1465-2080 1350-0872 |
| Popis: | Two conjugative plasmids (CPs) were isolated and characterized from the same ‘Sulfolobus islandicus’ strain, SOG2/4. The plasmids were separated from each other and transferred intoSulfolobus solfataricus. One has a high copy number and is not stable (pSOG1) whereas the other has a low copy number and is stably maintained (pSOG2). Plasmid pSOG2 is the firstSulfolobusCP found to have these characteristics. The genomes of both pSOG plasmids have been sequenced and were compared to each other and the availableSulfolobusCPs. Interestingly, apart from a very well-conserved core, 70 % of the pSOG1 and pSOG2 genomes is largely different and composed of a mixture of genes that often resemble counterparts in previously describedSulfolobusCPs. However, about 20 % of the predicted genes do not have known homologues, not even in other CPs. Unlike pSOG1, pSOG2 does not contain a gene for the highly conserved PlrA protein nor for obvious homologues of partitioning proteins. Unlike pNOB8 and pKEF9, both pSOG plasmids lack the so-called clustered regularly interspaced short palindrome repeats (CRISPRs). The sites of recombination between the two genomes can be explained by the presence of recombination motifs previously identified in otherSulfolobusCPs. Like otherSulfolobusCPs, the pSOG plasmids possess a gene encoding an integrase of the tyrosine recombinase family. This integrase probably mediates plasmid site-specific integration into the host chromosome at the highly conserved tRNAGluloci. |
| Databáze: | OpenAIRE |
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