Identification of Neuronal Lineages in the Drosophila Peripheral Nervous System with a 'Digital' Multi-spectral Lineage Tracing System
| Autor: | Dawen Cai, Macy W. Veling, Mike T. Veling, Christopher Litts, Hao Liu, Nigel S. Michki, Ye Li, Bing Ye |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Lineage (genetic) Multi spectral Computational biology Biology General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences 0302 clinical medicine Lineage tracing Peripheral Nervous System medicine Brainbow Animals Cell Lineage Drosophila (subgenus) lcsh:QH301-705.5 Neurons Brain biology.organism_classification 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) Peripheral nervous system Larva Identification (biology) Drosophila 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports, Vol 29, Iss 10, Pp 3303-3312.e3 (2019) Cell reports |
| ISSN: | 2211-1247 |
| Popis: | SUMMARY Elucidating cell lineages provides crucial understanding of development. Recently developed sequencing-based techniques enhance the scale of lineage tracing but eliminate the spatial information offered by conventional approaches. Multi-spectral labeling techniques, such as Brainbow, have the potential to identify lineage-related cells in situ. Here, we report nuclear Bitbow (nBitbow), a “digital” version of Brainbow that greatly expands the color diversity for scoring cells, and a suite of statistical methods for quantifying the lineage relationship of any two cells. Applying these tools to the Drosophila peripheral nervous system (PNS), we determined lineage relationship between all neuronal pairs. This study demonstrates nBitbow as an efficient tool for in situ lineage mapping, and the complete lineage relationship among larval PNS neurons opens new possibilities for studying how neurons gain specific features and circuit connectivity. In Brief Veling et al. report a multi-color labeling system and statistical methods for mapping cell lineages. They identify the lineage relationship of all neurons in the peripheral nervous system of Drosophila larvae and show the utility of this technique in mapping neurons in the CNS. Graphical Abstract |
| Databáze: | OpenAIRE |
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