Generation of a triple-fluorescent mouse strain allows a dynamic and spatial visualization of different liver phagocytes in vivo
| Autor: | Denise Carmona Cara, Érika de Carvalho, Brenda Naemi Nakagaki, Mônica Morais Santos, Bruna Araújo David, Rafael M. Rezende, Hortência Maciel de Castro-Oliveira, Rodrigo Pestana Lopes, Raquel Carvalho-Gontijo, Gustavo B. Menezes, Maria Alice Freitas-Lopes |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Receptors
CCR2 Cell CX3C Chemokine Receptor 1 gastroenterology Mice Transgenic Context (language use) Inflammation Biology Fluorescence Flow cytometry immunology Mice Immune system In vivo intravital microscopy medicine Animals lcsh:Science Receptor Microscopy Confocal Multidisciplinary medicine.diagnostic_test Flow Cytometry Phenotype CD11c Antigen Cell biology Mice Inbred C57BL medicine.anatomical_structure Liver fluorescent markers hepatology Immunology phagocytes lcsh:Q liver immunology medicine.symptom |
| Zdroj: | Anais da Academia Brasileira de Ciências v.91 suppl.1 2019 Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências (ABC) instacron:ABC Anais da Academia Brasileira de Ciências, Iss 0 (2017) Anais da Academia Brasileira de Ciências, Volume: 91 Supplement 1, Article number: e20170317, Published: 16 OCT 2017 LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV Anais da Academia Brasileira de Ciências, Issue: ahead, Published: 16 OCT 2017 |
| Popis: | Resident and circulating immune cells have been extensively studied due to their almost ubiquitous role in cell biology. Despite their classification under the “immune cell department”, it is becoming increasingly clear that these cells are involved in many different non-immune related phenomena, including fetus development, vascular formation, memory, social behavior and many other phenotypes. There is a huge potential in combining high-throughput assays - including flow cytometry and gene analysis - with in vivo imaging. This can improve our knowledge in both basic and clinical cell biology, and accessing the expression of markers that are relevant in the context of both homeostasis and disease conditions might be instrumental. Here we describe how we generated a novel mouse strain that spontaneously express three different fluorescence markers under control of well-studied receptors (CX3CR1, CCR2 and CD11c) that are involved in a plethora of stages of cell ontogenesis, maturation, migration and behavior. Also, we assess the percentage of the expression and co-expression of each marker under homeostasis conditions, and how these cells behave when a local inflammation is induced in the liver applying a cutting-edge technology to image cells by confocal intravital microscopy. |
| Databáze: | OpenAIRE |
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