Phosphatidylcholine could protect the defect of zearalenone exposure on follicular development and oocyte maturation
| Autor: | Na Li, Yan-Zhong Feng, Yong Zhao, Fang-Nong Lai, Xue-Lian Liu, Lan Li, Rui-Qian Zhang, Charles M. Nyachoti, Wei Shen |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
endocrine system Aging Swine Granulosa cell follicle lysophosphatidylcholine Andrology 03 medical and health sciences Follicle 0302 clinical medicine Ovarian Follicle Follicular antrum Follicular phase medicine Animals Platelet Activating Factor oocyte Antrum 030219 obstetrics & reproductive medicine Chemistry zearalenone fungi food and beverages Cell Biology Antral follicle Oocyte metabolomics Follicular fluid In Vitro Oocyte Maturation Techniques 030104 developmental biology medicine.anatomical_structure Oocytes Phosphatidylcholines Female Research Paper |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| Popis: | Zearalenone (ZEA) is a well-known exogenous endocrine disruptor and can lead to severe negative effects on the human and animal reproductive process. Using a follicle culture model, we have previously shown that ZEA exposure significantly affected the follicular development and antrum formation but the underlying mechanisms are not well known. Therefore, in this study, we explored the metabolomic changes of granulosa cell (GC) culture media with or without ZEA exposure. The results showed that ZEA significantly increased phosphatidylcholine or phosphatidyl ethanolamine adducts in culture medium. A comprehensive analysis with the metabolome data from follicular fluid of small and large antral follicles showed that lyso phosphatidylcholine (LPC) was accumulated during follicle growth, but was depleted by ZEA exposure. Exogenous supplement with LPC to the follicle growth media or oocyte maturation media can partly protect the defect of ZEA exposure on follicular antrum formation and oocyte maturation. Taken together, our results demonstrate that ZEA exposure hinders the follicular growth and exogenous LPC can practically protect the defect of ZEA on follicular development and oocyte maturation. |
| Databáze: | OpenAIRE |
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