Early specification and development of rabbit neural crest cells

Autor: Rebekah M. Charney, Erin Betters, Martín I. García-Castro
Rok vydání: 2018
Předmět:
0301 basic medicine
AP2α
Rabbit
Chick Embryo
SU5402
Medical and Health Sciences
FGF signaling
Neural crest
0302 clinical medicine
Mammalian embryogenesis
Neural crest formation
Cell Movement
Developmental
Neurulation
Pediatric
education.field_of_study
Gene Expression Regulation
Developmental
Embryo
Cell Differentiation
Biological Sciences
Biological Evolution
Cell biology
Neural Crest
AP2 alpha
embryonic structures
Vertebrates
Stem Cell Research - Nonembryonic - Non-Human
Rabbits
Sox9
Signal Transduction
Neural Tube
animal structures
1.1 Normal biological development and functioning
Neurogenesis
Sox10
SOX10
Population
Biology
Article
03 medical and health sciences
Underpinning research
Neural plate border
Animals
Humans
education
Molecular Biology
Body Patterning
Pax3
Neurosciences
Cell Biology
Gastrula
Stem Cell Research
Pax7
Gastrulation
Fibroblast Growth Factors
030104 developmental biology
Gene Expression Regulation
Neurula
Generic health relevance
PAX7
Specification
030217 neurology & neurosurgery
Developmental Biology
Transcription Factors
Zdroj: Developmental biology, vol 444 Suppl 1, iss Suppl 1
ISSN: 1095-564X
Popis: The phenomenal migratory and differentiation capacity of neural crest cells has been well established across model organisms. While the earliest stages of neural crest development have been investigated in non-mammalian model systems such as Xenopus and Aves, the early specification of this cell population has not been evaluated in mammalian embryos, of which the murine model is the most prevalent. Towards a more comprehensive understanding of mammalian neural crest formation and human comparative studies, we have used the rabbit as a mammalian system for the study of early neural crest specification and development. We examine the expression profile of well-characterized neural crest markers in rabbit embryos across developmental time from early gastrula to later neurula stages, and provide a comparison to markers of migratory neural crest in the chick. Importantly, we apply explant specification assays to address the pivotal question of mammalian neural crest ontogeny, and provide the first evidence that a specified population of neural crest cells exists in the rabbit gastrula prior to the overt expression of neural crest markers. Finally, we demonstrate that FGF signaling is necessary for early rabbit neural crest formation, as SU5402 treatment strongly represses neural crest marker expression in explant assays. This study pioneers the rabbit as a model for neural crest development, and provides the first demonstration of mammalian neural crest specification and the requirement of FGF signaling in this process.
Databáze: OpenAIRE
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