13-Methyl-palmatrubine induces apoptosis and cell cycle arrest in A549 cells in vitro and in vivo
| Autor: | Xiaoli Xu, Haixin Gou, XinGang Lu, Chunying Wang, Jingxian Chen, Bing Zhu, Chenghua Lu, Haizhu Xu, Wangchun Du |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Lung Neoplasms MAP Kinase Signaling System Berberine Alkaloids Cell ved/biology.organism_classification_rank.species Biology p38 Mitogen-Activated Protein Kinases Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Humans Cytotoxic T cell Cell Proliferation A549 cell ved/biology apoptosis Cell Cycle Checkpoints Articles General Medicine Cell cycle Xenograft Model Antitumor Assays Cell biology ErbB Receptors 030104 developmental biology medicine.anatomical_structure Oncology A549 Cells cell cycle arrest Apoptosis 030220 oncology & carcinogenesis Cancer cell 13-methyl-palmatrubine Corydalis yanhusuo EGFR-MAPK A431 cells |
| Zdroj: | Oncology Reports |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2016.5093 |
| Popis: | Corydalis yanhusuo, a well-known herbaceous plant, is commonly used in the treatment of inflammation, injury and pain. One natural agent isolated from Corydalis yanhusuo, 13-methyl-palmatrubine, was found to have a cytotoxic effect on cancer cells as reported in published studies. In the present study, we synthesized a potential anti-lung tumor agent, 13-methyl-palmatrubine and analyzed its activity. 13-Methyl-palmatrubine exhibited a cytotoxic effect on a panel of cancer cell lines in a time- and concentration-dependent manner. Among all the tested cancer cell lines, lung cancer A549 cells were most sensitive to 13-methyl-palmatrubine treatment. Meanwhile 13-methyl-palmatrubine showed less cytotoxicity in human normal cells. Our investigation revealed that 13-methyl-palmatrubine induced apoptosis and cell cycle arrest in A549 cells in a dose-dependent manner. Furthermore, 13-methyl-palmatrubine treatment caused activation of P38 and JNK pathways and blocked the EGFR pathway. In conclusion, our findings demonstrated that 13-methyl-palmatrubine inhibited the growth of A549 cells mediated by blocking of the EGFR signaling pathway and activation of the MAPK signaling pathway and provides a better understanding of the molecular mechanisms of 13-methyl-palmatrubine. |
| Databáze: | OpenAIRE |
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