Ploidy Level and Genetic Parameters for Phenotypic Traits in Bermudagrass (Cynodon spp.) Germplasm
| Autor: | Yolanda Lopez, Esteban F. Rios, Mario H. M. L. Andrade, Adina Y. Grossman, Kevin E. Kenworthy, Vânia Helena Techio, Marco Túlio Mendes Ferreira, Kevin Begcy, Ana Luisa Arantes Chaves |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Germplasm Cynodon forage breeding bermudagrass stem maggot Biology 01 natural sciences Crop 03 medical and health sciences Atherigona reversura Cultivar cvg Genome size polyploidy 030304 developmental biology 0303 health sciences flow cytometry cvg.computer_videogame food and beverages Agriculture Phenotypic trait biology.organism_classification Agronomy Ploidy Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Agronomy Volume 11 Issue 5 Agronomy, Vol 11, Iss 912, p 912 (2021) |
| ISSN: | 2073-4395 |
| DOI: | 10.3390/agronomy11050912 |
| Popis: | Bermudagrass (Cynodon spp.) is a forage and turf crop commonly used worldwide. The USDA bermudagrass germplasm set is composed of plant introductions (PI’s) collected around the world and contains different Cynodon species, primarily C. dactylon. The collection was screened in a replicated trial in Florida for forage yield, leaf width, nutritive value (NV), and Bermudagrass Stem Maggot (Atherigona reversura) (BSM), which is an invasive pest to the southeastern United States that damages bermudagrass fields. The goal of this research was to determine ploidy level and genome size in this USDA collection, and evaluate the influence of ploidy level in the estimation of genetic parameters for BSM, leaf width, dry matter yield, and NV traits. For chromosome counts using classical cytogenetics techniques, root tips and meristems were collected from a set of PI’s with known ploidy. The PI’s and cultivars with known chromosome counts were used as internal standards to run flow cytometry and estimate genome size of the PI’s with unknown ploidy. Ploidy level was determined for all accessions and were used to estimate genetic parameters of phenotypic traits. By providing information on ploidy levels and genetic parameters, this research will support breeding efforts and future selections for forage bermudagrass. |
| Databáze: | OpenAIRE |
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