Mud2 functions in transcription by recruiting the Prp19 and TREX complexes to transcribed genes
| Autor: | Sofia G. Georgieva, Katja Sträßer, D. V. Kopytova, Danny Misiak, Rashmi Minocha, Varvara Popova, Stefan Hüttelmaier |
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| Přispěvatelé: | Institute of Biochemistry |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins Transcription Genetic RNA Splicing Saccharomyces cerevisiae Computational biology Biology Saccharomyces 03 medical and health sciences Transcription (biology) Gene Expression Regulation Fungal ddc:570 Escherichia coli RNA and RNA-protein complexes Genetics Animals Drosophila Proteins Gene Cells Cultured RNA-Binding Proteins Phosphoproteins Splicing Factor U2AF Life sciences Drosophila melanogaster Exodeoxyribonucleases 030104 developmental biology Ribonucleoproteins Multiprotein Complexes RNA Splicing Factors Corrigendum Protein Binding |
| Zdroj: | Nucleic Acids Research 46(18):9749-9763 doi: 10.1093/nar/gky640 Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | The different steps of gene expression are intimately linked to coordinate and regulate this complex process. During transcription, numerous RNA-binding proteins are already loaded onto the nascent mRNA and package the mRNA into a messenger ribonucleoprotein particle (mRNP). These RNA-binding proteins are often also involved in other steps of gene expression than mRNA packaging. For example, TREX functions in transcription, mRNP packaging and nuclear mRNA export. Previously, we showed that the Prp19 splicing complex (Prp19C) is needed for efficient transcription as well as TREX occupancy at transcribed genes. Here, we show that the splicing factor Mud2 interacts with Prp19C and is needed for Prp19C occupancy at transcribed genes in Saccharomyces cerevisiae. Interestingly, Mud2 is not only recruited to intron-containing but also to intronless genes indicating a role in transcription. Indeed, we show for the first time that Mud2 functions in transcription. Furthermore, these functions of Mud2 are likely evolutionarily conserved as Mud2 is also recruited to an intronless gene and interacts with Prp19C in Drosophila melanogaster. Taken together, we classify Mud2 as a novel transcription factor that is necessary for the recruitment of mRNA-binding proteins to the transcription machinery. Thus, Mud2 is a multifunctional protein important for transcription, splicing and most likely also mRNP packaging. |
| Databáze: | OpenAIRE |
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