Collection and Rearing of Container Mosquitoes and a 24-h Addition to the CDC Bottle Bioassay
| Autor: | Casey Parker |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
AcademicSubjects/SCI01382
0106 biological sciences Mosquito Control business.product_category 030231 tropical medicine container mosquito Mosquito Vectors Aedes aegypti Container (type theory) 01 natural sciences CDC bottle bioassay Specimen Handling resistance Toxicology 03 medical and health sciences 0302 clinical medicine Aedes parasitic diseases Bottle Animals Bioassay biology Special Collection: Protocols in Medical and Veterinary Entomology fungi General Medicine rearing biology.organism_classification Disease control Resistance monitoring 010602 entomology Insect Science business Entomology Protocols |
| Zdroj: | Journal of Insect Science |
| ISSN: | 1536-2442 |
| DOI: | 10.1093/jisesa/ieaa059 |
| Popis: | Container mosquitoes (Diptera: Culicidae) oviposit their eggs in both natural and artificial containers. Many container mosquito species also serve as important vectors of disease-causing pathogens including Aedes aegypti, Ae. albopictus, and Ae. triseriatus. Control of these species can be done through the use of adulticide sprays. The efficacy of these treatments is highly dependent on the insecticide susceptibility status of the local mosquito populations. This paper provides protocols on collecting and rearing container mosquitoes for use in the Centers for Disease Control and Prevention (CDC) bottle bioassay. A brief description of the CDC bottle bioassay is provided as well as a standardized protocol for the incorporation of a 24-h mortality to the CDC bottle bioassay. Results from this 24-h holding addition to the CDC bottle bioassay reveal that some forms of resistance may be missed without the incorporation of the additional mortality reading. These protocols provide a foundation for new laboratories to establish rearing protocols and begin conducting resistance monitoring. |
| Databáze: | OpenAIRE |
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