Nanocomposite Treatment Reduces Disease and Lethality in a Murine Model of Acute Graft-versus-Host Disease and Preserves Anti-Tumor Effects
Autor: | Bárbara M. Rezende, William Antonio Gonçalves, Priscila T. T. Bernardes, Marina G. M. Castor, Talles Prosperi de Paula, Vanessa Pinho, Elias Gonzaga Vieira, Danielle G. Souza, Maurício V. B. Pinheiro, Alesandra C. Reis, Carolina B. Resende, Mauro M. Teixeira |
---|---|
Rok vydání: | 2015 |
Předmět: |
Chemokine
Neutrophils medicine.medical_treatment Graft vs Host Disease lcsh:Medicine CCL3 Antineoplastic Agents Inflammation Liver transplantation Nanocomposites Pathogenesis Mice Peritoneal cavity Cell Line Tumor medicine Animals lcsh:Science Bone Marrow Transplantation Gastrointestinal tract Multidisciplinary biology business.industry Macrophages lcsh:R medicine.disease Mice Inbred C57BL Disease Models Animal surgical procedures operative Graft-versus-host disease medicine.anatomical_structure Liver Mice Inbred DBA Immunology biology.protein lcsh:Q Fullerenes medicine.symptom Reactive Oxygen Species business Research Article |
Zdroj: | PLoS ONE, Vol 10, Iss 4, p e0123004 (2015) PLoS ONE |
ISSN: | 1932-6203 |
DOI: | 10.1371/journal.pone.0123004 |
Popis: | Graft versus host disease (GVHD) is an immunological disorder triggered by bone marrow transplantation that affects several organs, including the gastrointestinal tract and liver. Fullerenes and their soluble forms, fullerols, are nanocomposites with a closed symmetrical structure with anti-inflammatory and anti-oxidant properties. The present study evaluated the effects of treatment with the fullerol (C60(OH)18-20) in the development and pathogenesis of GVHD in a murine model. Mice with experimental GVHD that were treated with the fullerol showed reduced clinical signs of disease and mortality compared with untreated mice. Treatment with the fullerol decreased the hepatic damage associated with reduced hepatic levels of reactive oxygen species, pro-inflammatory cytokines and chemokines (IFN-γ TNF-α, CCL2, CCL3 and CCL5) and reduced leukocyte accumulation. The amelioration of GVHD after treatment with the fullerol was also associated with reduced intestinal lesions and consequent bacterial translocation to the blood, liver and peritoneal cavity. Moreover, the fullerol treatment alleviated the GVHD while preserving effects of the graft against a leukemia cell line (GFP+P815). In summary, the fullerol was effective in reducing the GVHD inflammatory response in mice and may suggest novel ways to treat this disease. |
Databáze: | OpenAIRE |
Externí odkaz: |