| Autor: |
Erez, Amir, Mukherjee, Ratnadeep, Day, Alexandre, Mehta, Pankaj, Altan-Bonnet, Grégoire |
| Rok vydání: |
2019 |
| Předmět: |
|
| DOI: |
10.1101/552489 |
| Popis: |
We present a new method to directly quantify the dynamics of differentiation of multiple cellular subsets in unperturbed mice. We combine a pulse-chase protocol of IdU injections with subsequent analysis by mass cytometry (CyTOF), and mathematical modeling of the IdU dynamics. Measurements by CyTOF allow for a wide range of cells to be analyzed at once, due to the availability of a large staining panel without the complication of fluorescence spill-over. These are also compatible with direct detection of integrated iodine signal, with minimal impact on immunophenotyping based on surface markers. Mathematical modeling beyond a binary classification of surface marker abundance allows for a continuum of cellular states as the cells transition from one state to another. Thus, we present a complete and robust method for directly quantifying differentiation at the systemic level, allowing for system-wide comparisons between different mouse strains and/or experimental conditions. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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