Cell Cl and transepithelial Na transport in toad urinary bladder
| Autor: | A. G. Butt, J. M. Bowler, Robert D. Purves, C. W. McLaughlin, Anthony D. C. Macknight |
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| Rok vydání: | 1994 |
| Předmět: |
Intracellular Fluid
4-Acetamido-4'-isothiocyanatostilbene-2 2'-disulfonic Acid Physiology Urinary Bladder Cell Cell volume Biophysics Toad Gluconates Antiporters Epithelium Membrane Potentials X ray microanalysis Amiloride Chlorides biology.animal medicine Animals Chloride-Bicarbonate Antiporters Bumetanide Cell Size Urinary bladder biology Chemistry Sodium Epithelial Cells Cell Biology Human physiology Molecular biology Culture Media Hydrochlorothiazide medicine.anatomical_structure Biochemistry Bendroflumethiazide Nitrobenzoates Bufo marinus Electron Probe Microanalysis medicine.drug |
| Zdroj: | The Journal of Membrane Biology. 142 |
| ISSN: | 1432-1424 0022-2631 |
| DOI: | 10.1007/bf00233379 |
| Popis: | Relationships between short-circuit current (I sc), cell Cl and the mechanism(s) of Cl accumulation in toad bladder epithelial cells were investigated. In serosal Cl-free gluconate Ringer, 80% of the cell Cl (measured by x-ray microanalysis) was lost over 30–60 min with an associated decrease in cell water content. Concomitantly, I sc fell to 20% of its initial value within 10 min but then recovered to 45% of its initial value despite continued Cl loss. With the reintroduction of Cl, cell Cl and I sc both recovered within 10 min. Serosal SITS (4 acetamido-4′-isothiocyano-stilbene-2,2′-disulfonate; 0.5 mm) plus bumetanide (0.1 mm), did not prevent the fall in I sc or the loss of cell Cl in gluconate medium, although they did inhibit subsequent recovery of I sc in this medium. They also prevented the recovery of I sc in Cl medium but not the reaccumulation of Cl by the cells. Although SITS and bumetanide did not prevent the loss or recovery of Cl, they modified the pattern of the ion changes. In their absence, changes in cellular Cl were twice that of the changes in measured cellular cations implicating basolateral Cl/HCO3 exchange in Cl movement. With SITS plus bumetanide present, changes of similar magnitude in Cl were associated with equivalent changes in cation, consistent with the inhibition of Cl/ HCO3 exchange. |
| Databáze: | OpenAIRE |
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