| Autor: |
Hong Ye, Jiongwei Pan, Xiaoping Cai, Zhangyong Yin, Lu Li, Enhui Gong, Cunlai Xu, Hao Zheng, Zhuo Cao, Enguo Chen, Junfeng Qian |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Experimental and therapeutic medicine. 24(2) |
| ISSN: |
1792-1015 |
| Popis: |
Interstitial pneumonia is a pulmonary interstitial inflammatory and fibrosis disease with a variety of causes that causes respiratory disorders and threatens the lives of patients. The present study aimed to investigate the expression of interleukin (IL)-10 in peripheral blood of patients with interstitial pneumonia and its biological functions in pulmonary fibroblasts. A total of 42 patients with idiopathic pulmonary fibrosis (IPF) and 20 healthy subjects were included. ELISA was used to determine IL-10 concentration in serum from the patients and healthy subjects. Primary fibroblasts were isolated from lung tissue successfully and determined by morphology. The CCK-8 assay was performed to determine the effect of IL-10 expression on cell viability. Western blotting was used to determine COL1a1, COL1a2 and IL-10R1 protein expression. Flow cytometry was used for cell cycle analysis and to determine the number of IL-10 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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