Coenzyme Q10 supplementation of human oocyte in vitro maturation reduces postmeiotic aneuploidies
| Autor: | X. Johné Liu, Long Ma, Jiazi Xie, Jiayin Liu, Yan Xing, Jing Wang, Lingbo Cai, Jiandong Shen, Yugui Cui, Meng-Ting Hu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Ubiquinone Aneuploidy Fertilization in Vitro Biology Andrology 03 medical and health sciences chemistry.chemical_compound Polar body 0302 clinical medicine Human fertilization medicine Humans Coenzyme Q10 030219 obstetrics & reproductive medicine Obstetrics and Gynecology Chromosome Middle Aged medicine.disease Oocyte In vitro maturation Culture Media In Vitro Oocyte Maturation Techniques Meiosis 030104 developmental biology medicine.anatomical_structure Fertility Reproductive Medicine chemistry Infertility Oocytes Laboratory observation Female Maternal Age |
| Zdroj: | Fertility and sterility. 114(2) |
| ISSN: | 1556-5653 |
| Popis: | Objective To evaluate the effect of coenzyme Q10 (CoQ10) supplementation on oocyte maturation rates and postmeiotic aneuploidy rates during in vitro maturation (IVM) of human oocytes. Design Clinical laboratory observation. Setting Hospital and university laboratories. Patient(s) Forty-five patients aged ≥38 years and 18 patients aged ≤30 years undergoing in vitro fertilization. Intervention(s) The germinal vesicle–stage oocytes and associated cumulus cells were cultured in IVM media for 24–48 hours with or without 50 μmol/L CoQ10. Oocyte maturation rates were determined based on the presence or absence of the first polar body. Postmeiotic aneuploidies were determined using next-generation sequencing analyses of biopsied polar bodies. Main Outcome Measure(s) Oocyte maturation rates, postmeiotic oocyte aneuploidy rates, and chromosome aneuploidy frequencies. Result(s) In women aged 38–46 years, 50 μmol/L CoQ10 significantly increased oocyte maturation rates (82.6% vs. 63.0%; P=.035), reduced oocyte aneuploidy rates (36.8% vs. 65.5%; P=.020), and reduced chromosome aneuploidy frequencies (4.1% vs. 7.0%; P=.012. In women aged ≤30 years, we failed to demonstrate an effect of CoQ10 on oocyte maturation rates or postmeiotic aneuploidies. Conclusion(s) CoQ10 supplementation during IVM increased oocyte maturation rates and reduced postmeiotic aneuploidies for older women. |
| Databáze: | OpenAIRE |
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