Athabasca Oil Sands Petcoke Extract Elicits Biochemical and Transcriptomic Effects in Avian Hepatocytes
| Autor: | Kim L. Williams, Yifeng Zhang, Jonathan W. Martin, Suzanne Chiu, Doug Crump |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Mrna expression Fraction (chemistry) 010501 environmental sciences Ecotoxicology 01 natural sciences Birds Transcriptome 03 medical and health sciences chemistry.chemical_compound Cytochrome P-450 CYP1A1 Animals Environmental Chemistry Oil and Gas Fields Petroleum Pollution Coke 0105 earth and related environmental sciences Waste management Dimethyl sulfoxide Gene Expression Profiling Petroleum coke General Chemistry Solvent Petroleum 030104 developmental biology chemistry Environmental chemistry Hepatocytes Oil sands |
| Zdroj: | Environmental Science & Technology. 51:5783-5792 |
| ISSN: | 1520-5851 0013-936X |
| Popis: | Petroleum coke or "petcoke" is a granular carbonaceous material produced during the upgrading of heavy crude oils, including bitumen. Petcoke dust was recently reported as an environmental contaminant in the Athabasca oil sands region, but the ecotoxicological hazards posed by this complex bitumen-derived material-including those to avian species-have not been characterized. In this study, solvent extracts (x) of delayed and fluid petcoke (xDP and xFP) were prepared and dissolved in dimethyl sulfoxide. A water-accommodated fraction of delayed petcoke (waDP) was also prepared. Graded concentrations of xDP, xFP, and waDP were administered to chicken and double-crested cormorant hepatocytes to determine effects on 7-ethoxyresorufin-O-deethylase (EROD) activity, porphyrin accumulation, and mRNA expression. Polycyclic aromatic compounds (PACs) were characterized, and xDP, xFP, and waDP had total PAC concentrations of 93 000, 270, and 5.3 ng/mL. The rank order of biochemical and transcriptomic responses was xDPxFPwaDP (e.g., EROD EC |
| Databáze: | OpenAIRE |
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