Transcriptomic Analysis of the Anthocyanin Biosynthetic Pathway Reveals the Molecular Mechanism Associated with Purple Color Formation in Dendrobium Nestor

Autor:	Huang Changyan, Tang Xuan, Cui Xueqiang, Li Xianmin, Zhang Zibin, Li Xiuling, Lu Jiashi, Deng Jieling
Rok vydání:	2021
Předmět:	0106 biological sciences 0301 basic medicine Chalcone synthase anthocyanin biosynthesis Phenylalanine ammonia-lyase 01 natural sciences Article General Biochemistry Genetics and Molecular Biology Dendrobium 03 medical and health sciences chemistry.chemical_compound gene coexpression transcription factors Brassinosteroid pigmentation MYB orchid lcsh:Science Ecology Evolution Behavior and Systematics Illumina dye sequencing biology fungi Structural gene food and beverages Paleontology biology.organism_classification 030104 developmental biology Biochemistry chemistry Space and Planetary Science biology.protein lcsh:Q Petal 010606 plant biology & botany
Zdroj:	Life, Vol 11, Iss 113, p 113 (2021) Life Volume 11 Issue 2
ISSN:	2075-1729
DOI:	10.3390/life11020113
Popis:	Dendrobium nestor is a famous orchid species in the Orchidaceae family. There is a diversity of flower colorations in the Dendrobium species, but knowledge of the genes involved and molecular mechanism underlying the flower color formation in D. nestor is less studied. Therefore, we performed transcriptome profiling using Illumina sequencing to facilitate thorough studies of the purple color formation in petal samples collected at three developmental stages, namely—flower bud stage (F), half bloom stage (H), and full bloom stage (B) in D. nestor. In addition, we identified key genes and their biosynthetic pathways as well as the transcription factors (TFs) associated with purple flower color formation. We found that the phenylpropanoid–flavonoid–anthocyanin biosynthesis genes such as phenylalanine ammonia lyase, chalcone synthase, anthocyanidin synthase, and UDP-flavonoid glucosyl transferase, were largely up-regulated in the H and B samples as compared to the F samples. This upregulation might partly account for the accumulation of anthocyanins, which confer the purple coloration in these samples. We further identified several differentially expressed genes related to phytohormones such as auxin, ethylene, cytokinins, salicylic acid, brassinosteroid, and abscisic acid, as well as TFs such as MYB and bHLH, which might play important roles in color formation in D. nestor flower. Sturdy upregulation of anthocyanin biosynthetic structural genes might be a potential regulatory mechanism in purple color formation in D. nestor flowers. Several TFs were predicted to regulate the anthocyanin genes through a K-mean clustering analysis. Our study provides valuable resource for future studies to expand our understanding of flower color development mechanisms in D. nestor.
Databáze:	OpenAIRE
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