Trehalose-6-phosphate synthetase activity in extracts of Dictyostelium discoideum
| Autor: | S. Alexander, Maurice Sussman |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Uridine Diphosphate Glucose
Hot Temperature Trehalose-6-phosphate Cold sensitive Cell Biology Biology biology.organism_classification Dictyostelium discoideum Ammonium sulfate fractionation Synthetase activity Cold Temperature Biochemistry Glucosyltransferases Biological Assay Dictyostelium Myxomycetes Molecular Biology Developmental Biology |
| Zdroj: | Developmental Biology. 46:211-215 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/0012-1606(75)90098-6 |
| Popis: | Trehalose-6-phosphate (T-6-P) synthetase activity in extracts of Dictyostelium discoideum has been reexamined in an effort to resolve discrepancies between the results of previous studies ( R. Roth and M. Sussman (1966) . Biochim. Biophys. Acta , 122, 225; K. A. Killick and B. E. Wright (1972) . J. Biol. Chem. , 247, 2967). We find that T-6-P synthetase is not cold sensitive as reported by Killick and Wright (1972) , is not present in bacterial-grown vegetative cells (though subject to some modulation by other nutritional conditions), and is not in our hands unmasked or activated by ammonium sulfate fractionation. We conclude that the pattern of T-6-P synthetase accumulation and disappearance during fruiting body construction in D. discoideum is as originally described by R. Roth and M. Sussman (1968) . J. Biol. Chem. , 243, 5081) and confirmed elsewhere ( P. C. Newell et al. (1972) . J. Mol. Biol. , 63, 373; R. W. Brackenbury et al. (1974) . J. Mol. Biol. , 90, 529; B. D. Hames and J. M. Ashworth (1974) . Biochem. J. , 142, 301). |
| Databáze: | OpenAIRE |
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