SARS-CoV-2 Detection with De Novo-Designed Synthetic Riboregulators
| Autor: | Urartu Ozgur Safak Seker, Recep Erdem Ahan, Sila Köse, Hurrem Bodur, İlkay Çisil Köksaldı, Aliye Bastug, Nedim Haciosmanoglu, Aykut Ozkul, Murat Alp Güngen, Bedia Dinç, Ebru Şahin Kehribar |
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| Přispěvatelé: | Köksaldı, İlkay Çisil, Köse, Sıla, Ahan, Recep Erdem, Hacıosmanoğlu, Nedim, Şahin Kehribar, Ebru, Güngen, Murat Alp, Şeker, Urartu Özgür Şafak |
| Rok vydání: | 2021 |
| Předmět: |
2019-20 coronavirus outbreak
Coronavirus disease 2019 (COVID-19) SARS-CoV-2 Sensors Chemistry Point-of-Care Systems Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) fungi 010401 analytical chemistry COVID-19 Testing and assessment Genomics Disease Computational biology 010402 general chemistry 01 natural sciences Article World health 0104 chemical sciences Analytical Chemistry Synthetic biology Genetics Humans Pandemics |
| Zdroj: | Analytical Chemistry |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.1c00886 |
| Popis: | SARS-CoV-2 is a human pathogen and the main cause of COVID-19 disease, announced as a global pandemic by the World Health Organization. COVID-19 is characterized by severe conditions, and early diagnosis can make dramatic changes for both personal and public health. Low-cost, easy-to-use diagnostic capabilities can have a very critical role in controlling the transmission of the disease. Here, we are reporting a state-of-the-art diagnostic tool developed with an in vitro synthetic biology approach by employing engineered de novo riboregulators. Our design coupled with a home-made point-of-care device can detect and report the presence of SARS-CoV-2-specific genes. The presence of SARS-CoV-2-related genes triggers the translation of sfGFP mRNAs, resulting in a green fluorescence output. The approach proposed here has the potential of being a game changer in SARS-CoV-2 diagnostics by providing an easy-to-run, low-cost diagnostic capability. |
| Databáze: | OpenAIRE |
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