Novel Determinants of Intestinal Colonization of Salmonella enterica Serotype Typhimurium Identified in Bovine Enteric Infection
| Autor: | Hee-Jeong Yang, Johanna R. Elfenbein, Jinbai Guo, Tiana Endicott-Yazdani, Steffen Porwollik, Sara D. Lawhon, Michael McClelland, Aimee Maple, Katharine D. Andrews, Yury Ragoza, Lydia M. Bogomolnaya, Kimberly DeAtley, Marissa Talamantes, Ping Cui, Helene Andrews-Polymenis, Pui Cheng, Yi Zheng, Tyler Tatsch, Christine Shields |
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| Rok vydání: | 2013 |
| Předmět: |
Salmonella typhimurium
Salmonella Virulence Factors Immunology Mutant Cattle Diseases Virulence Salmonella infection Biology medicine.disease_cause Microbiology Medicine and Health Sciences medicine Animals Genetic Testing Gene Salmonella Infections Animal Genetic Complementation Test medicine.disease biology.organism_classification Molecular Pathogenesis Phenotype Gastroenteritis Complementation Disease Models Animal Infectious Diseases Salmonella enterica Cattle Parasitology Gene Deletion |
| Zdroj: | Elfenbein, Johanna R.; Endicott-Yazdani, Tiana; Porwollik, Steffen; Bogomolnaya, Lydia M.; Cheng, Pui; Guo, Jinbai; et al.(2013). Novel Determinants of Intestinal Colonization of Salmonella enterica Serotype Typhimurium Identified in Bovine Enteric Infection. Infection and Immunity, 81(11), 4311-4320. UC Irvine: Institute for Clinical and Translational Science. Retrieved from: http://www.escholarship.org/uc/item/18d1536k |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.00874-13 |
| Popis: | Cattle are naturally infected with Salmonella enterica serotype Typhimurium and exhibit pathological features of enteric salmonellosis that closely resemble those in humans. Cattle are the most relevant model of gastrointestinal disease resulting from nontyphoidal Salmonella infection in an animal with an intact microbiota. We utilized this model to screen a library of targeted single-gene deletion mutants to identify novel genes of Salmonella Typhimurium required for survival during enteric infection. Fifty-four candidate mutants were strongly selected, including numerous mutations in genes known to be important for gastrointestinal survival of salmonellae. Three genes with previously unproven phenotypes in gastrointestinal infection were tested in bovine ligated ileal loops. Two of these mutants, STM3602 and STM3846 , recapitulated the phenotype observed in the mutant pool. Complementation experiments successfully reversed the observed phenotypes, directly linking these genes to the colonization defects of the corresponding mutant strains. STM3602 encodes a putative transcriptional regulator that may be involved in phosphonate utilization, and STM3846 encodes a retron reverse transcriptase that produces a unique RNA-DNA hybrid molecule called multicopy single-stranded DNA. The genes identified in this study represent an exciting new class of virulence determinants for further mechanistic study to elucidate the strategies employed by Salmonella to survive within the small intestines of cattle. |
| Databáze: | OpenAIRE |
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