Molecular Detection of Dopamine Decarboxylase Expression by Means of Reverse Transcriptase and Polymerase Chain Reaction in Bone Marrow and Peripheral Blood
| Autor: | Daniela Polastri, Fabio Bozzi, Elena Barzanò, Franca Fossati-Bellani, Marta Podda, Paola Collini, Felicita Gambirasio, Bruno Brando, Roberto Luksch |
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| Rok vydání: | 2004 |
| Předmět: |
Tyrosine 3-Monooxygenase
Pathology and Forensic Medicine Flow cytometry Neuroblastoma Bone Marrow Cell Line Tumor Biomarkers Tumor medicine Humans RNA Messenger Molecular Biology Tumor marker integumentary system medicine.diagnostic_test Tyrosine hydroxylase Reverse Transcriptase Polymerase Chain Reaction business.industry fungi Infant Cell Biology Flow Cytometry medicine.disease Molecular biology Minimal residual disease CD56 Antigen Reverse transcriptase Real-time polymerase chain reaction medicine.anatomical_structure Child Preschool Dopa Decarboxylase Bone marrow business |
| Zdroj: | Diagnostic Molecular Pathology. 13:135-143 |
| ISSN: | 1052-9551 |
| Popis: | A highly sensitive molecular method was used to evaluate the presence of dopamine decarboxylase (DDC) mRNA in the bone marrow and peripheral blood of patients with neuroblastoma (NB). DDC, like tyrosine hydroxylase (TH), is an enzyme involved in the catecholamine synthesis pathway and has recently been proposed as a specific marker of NB among pediatric malignancies. DDC transcript was detected in five of five NB cell lines, 10 of 10 NB primary tumors, 17 of 18 (94%) bone marrow samples, and 12 of 18 (66%) blood samples drawn at diagnosis in 18 patients affected by disseminated NB. In contrast, no PCR signal was found in 20 bone marrow samples obtained from patients with other malignancies or in eight of nine marrow and blood samples drawn from patients with localized NB (two stage 2 and seven stage 3). In addition, all marrow and blood samples obtained from NB patients at relapse revealed DDC mRNA. Furthermore, the percentage of DDC-positive samples was lower among the samples drawn from these patients during treatment. By comparison with conventional methods for disease evaluation, DDC transcript research can increase the sensitivity of NB cell detection in marrow and blood samples at diagnosis and during the treatment and follow-up of NB patients. These results suggest that finding DDC mRNA in NB patients could be a potential marker for minimal residual disease study. |
| Databáze: | OpenAIRE |
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