Development of a hexavalent recombinant protein vaccine adjuvanted with Montanide ISA 50 V and determination of its protective efficacy against acute toxoplasmosis
| Autor: | Remziye Deveci, Aysu Değirmenci Döşkaya, Mina Kalantari-Dehaghi, Mert Döşkaya, Sultan Gülçe İz, Adnan Yüksel Gürüz, Esra Atalay Şahar, Hüseyin Can |
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| Přispěvatelé: | Ege Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Protozoan Vaccines medicine.medical_treatment 030231 tropical medicine Protozoan Proteins Toxoplasma gondii Montanide ISA 50V Enzyme-Linked Immunosorbent Assay Epitope law.invention lcsh:Infectious and parasitic diseases 03 medical and health sciences Epitopes Mice 0302 clinical medicine Immune system Antigen Adjuvants Immunologic law medicine Escherichia coli Vaccines DNA Animals lcsh:RC109-216 Mannitol Adjuvant Montanide ISA 50 V Immunity Cellular biology Hexavalant Recombinant protein vaccine medicine.disease biology.organism_classification Virology Toxoplasmosis Recombinant Proteins Immunity Humoral 030104 developmental biology Infectious Diseases Immunoglobulin G Recombinant DNA Female Toxoplasma CD8 Research Article |
| Zdroj: | BMC Infectious Diseases BMC Infectious Diseases, Vol 20, Iss 1, Pp 1-16 (2020) |
| ISSN: | 1471-2334 |
| Popis: | BackgroundToxoplasma gondii is an obligate intracellular parasite that can infect almost all warm-blooded animals, avian species and humans. Toxoplasmosis is asymptomatic in healthy individuals, whereas it may lead to death in immune suppressed or deficient patients. A vaccine against T. gondii is required to prevent consequences of the infection. the aim of this study is to generate a multivalent recombinant protein vaccine against T. gondii.Methods49 previously discovered antigenic proteins of T gondii were evaluated by their expression level in E. coli and by comprehensive bioinformatics analyses to determine antigenic epitopes. Based on these analyses, six vaccine candidate proteins were selected to generate a hexavalent recombinant protein vaccine adjuvanted with Montanide ISA 50V. Humoral and cellular immune responses were determined by flow cytometry and ELISA. Vaccinated mice were challenged with T. gondii Ankara strain tachyzoites.ResultsIn mice vaccinated with hexavalent vaccine, strong total IgG (P Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [110S200] This study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) grant 110S200 to AYG. the funding bodies played no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript. |
| Databáze: | OpenAIRE |
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