Radiolysis of chromatin extracted from cultured mammalian cells: production of alkali-labile strand damage in DNA
| Autor: | L. K. Mee, Stephen Adelstein, G. Stein |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Sucrose
Aqueous solution Radical DNA Single-Stranded Dose-Response Relationship Radiation General Medicine Biology In Vitro Techniques Molecular biology Chromatin Cell Line chemistry.chemical_compound chemistry Cesium Radioisotopes Gamma Rays Yield (chemistry) Radiolysis Animals A-DNA Cobalt Radioisotopes DNA |
| Zdroj: | International journal of radiation biology and related studies in physics, chemistry, and medicine. 33(5) |
| ISSN: | 0020-7616 |
| Popis: | Chromatin has been isolated from cultured Chinese-hamster lung fibroblasts as an expanded aqueous gel. The DNA in isolated chromatin has been examined by sedimentation on alkaline sucrose gradients. The average molecular weight of the DNA has been determined to be 50 million. gamma-irradiation of isolated chromatin degrades the DNA to lower molecular weight. The yield of single-strand breaks in the DNA is 0.02 single-strand breaks per krad-10(6) dalton, calculated from a dose-range of--400 krad and covering a DNA molecular weight range of 2 X 10(7)-1.4 X 10(5). There is a considerable difference in the efficiency of the formation of single-strand breaks in DNA irradiated as isolated chromatin compared with chromatin irradiated in whole cells before isolation. For isolated chromatin, values of 6 dV per break have been calculated compared with about 80 eV per break for chromatin irradiated in whole cells, which suggest a large contribution from indirect action by aqueous radicals in isolated chromatin. |
| Databáze: | OpenAIRE |
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