Histomorphological changes in murine fibrosarcoma after hypericin-based photodynamic therapy
| Autor: | Pavol Miškovský, Ján Štubňa, Ivan Čavarga, Silvia Rybárová, Ladislav Mirossay, Nikita Bobrov, Peter Fedoročko, Brezáni P, František Longauer |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Pathology medicine.medical_specialty medicine.medical_treatment Fibrosarcoma Pharmaceutical Science Inflammation Photodynamic therapy Murine fibrosarcoma Necrotic Change Radiation Dosage chemistry.chemical_compound Mice Cell Line Tumor Drug Discovery medicine Animals Humans Photosensitizer Perylene Pharmacology Anthracenes Mice Inbred C3H Photosensitizing Agents business.industry Plant Extracts medicine.disease Thrombosis Antineoplastic Agents Phytogenic Hypericin Disease Models Animal Complementary and alternative medicine chemistry Photochemotherapy Molecular Medicine medicine.symptom business Hypericum Injections Intraperitoneal Phytotherapy |
| Zdroj: | Phytomedicine : international journal of phytotherapy and phytopharmacology. 14(2-3) |
| ISSN: | 0944-7113 |
| Popis: | Histomorphological changes in murine fibrosarcoma after photodynamic therapy (PDT) based on the natural photosensitizer hypericin were evaluated. C3H/DiSn mice were inoculated with fibrosarcoma G5:1:13 cells. When the tumour reached a volume of 40–80 mm3 the mice were intraperitoneally injected with hypericin, either in a single dose (5 mg/kg; 1 or 6 h before laser irradiation) or two fractionated doses (2.5 mg/kg; 6 and 1 h before irradiation with laser light; 532 nm, 70 mW/cm2, 168 J/cm2). All groups of PDT-treated animals with single and fractionated hypericin dosing presented primary vascular reactions including vascular dilatation, congestion, thrombosis and oedema. Two hours after PDT there were necrotic changes with small, rather focal appearance. One day after therapy the necrotic areas were enhanced, often affecting a complete superficial layer of tumour tissue. Necrotic areas were accompanied with inflammation and haemorrhages. |
| Databáze: | OpenAIRE |
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