The excretion of five different 2-hydroxyoestrogen monomethyl ethers in human pregnancy urine
| Autor: | H.P. Gelbke, R. Knuppen |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Methyl Ethers
Oxidative Demethylation Catechol Chromatography Gas Chromatography Estradiol Chromatography Paper Estriol Phenazine Estrogens Ether Urine Biochemistry Excretion chemistry.chemical_compound Endocrinology chemistry Pregnancy Metabolic effects Humans Organic chemistry Female Hydroxysteroids |
| Zdroj: | Journal of Steroid Biochemistry. 7:457-463 |
| ISSN: | 0022-4731 |
| DOI: | 10.1016/0022-4731(76)90112-6 |
| Popis: | The excretion pattern of different 2-hydroxyoestrogen monomethyl ethers (2-methoxyoestrone, 2-hydroxyoestrone 3-methyl ether, 2-methoxyoestradiol, 2-hydroxyoestradiol 3-methyl ether and the sum of the isomeric monomethyl ethers of 2-hydroxyoestriol) was quantitatively determined in urines of pregnant women. The analytical procedure involved several chromatographic purification steps and microchemical reactions, i.e. oxidative demethylation of the monomethyl ethers to the catechol compounds (2-hydroxyoestrone, 2-hydroxyoestradiol and 2-hydroxyoestriol) and conversion of 2-hydroxyoestrone and 2-hydroxyoestradiol to the corresponding phenazine derivatives. The phenazines were quantitated by U.V.-spectrometry and 2-hydroxyoestriol was determined by gas-chromatography. The specificity of the procedure was established by various spectrometric, chromatographic and microchemical methods. The amounts of the different oestrogens varied within the following ranges: 2-methoxyoestrone = 20–360 μg/24 h; 2-hydroxyoestrone 3-methyl ether = 5–50 μg/24 h; 2-methoxyoestradiol = |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|