Determination of Free Amino Acids in Bee Pollen by Liquid Chromatography with Fluorescence Detection

Autor: Ana María Ares Sacristán, Mª Teresa Martín Gómez, Laura Toribio, JOSÉ BERNAL DEL NOZAL
Rok vydání: 2022
Předmět:
Zdroj: UVaDOC. Repositorio Documental de la Universidad de Valladolid
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ISSN: 1936-976X
1936-9751
Popis: Producción Científica
Bee pollen is one of the hive products that is of most interest today due to its multiple beneficial health properties, making it an increasingly popular food supplement. Bee pollen contains many bioactive compounds, such as fatty acids, vitamins, minerals, proteins, and amino acids, among others. In the present study, the free amino acid content was determined in bee pollen by using liquid chromatography coupled to a fluorescence detector. Sample treatment consisted of a solvent extraction of the free amino acids with ultrapure water and a further centrifugation of the extract, which was repeated twice. After that, it was necessary to perform a pre-column derivatization of the amino acids using a combination of two reagents (o-phthalaldehyde and 9-fluorenylmethyl chloroformate) prior to their separation in a Gemini® C18 reverse phase column in gradient elution mode. The analytical performance was evaluated, and several commercial bee pollen samples were analyzed. Significant differences in the free amino acid profile and concentration, which ranged between 19 and 192 mg/g, were observed depending on the botanical origin of the samples.
Ministerio de Economía y Competitividad y el Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (project number RTA 2015–00013-C03-03)
Publicación en abierto financiada por el Consorcio de Bibliotecas Universitarias de Castilla y León (BUCLE), con cargo al Programa Operativo 2014ES16RFOP009 FEDER 2014-2020 DE CASTILLA Y LEÓN, Actuación:20007-CL - Apoyo Consorcio BUCLE
Databáze: OpenAIRE
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