Root exudate of Solanum tuberosum is enriched in galactose-containing molecules and impacts the growth of Pectobacterium atrosepticum
| Autor: | Maïté Vicré-Gibouin, Abdoul Salam Koroney, Carole Plasson, Barbara Pawlak, Azeddine Driouich, Ramatou Sidikou, Laurence Menu-Bouaouiche |
|---|---|
| Přispěvatelé: | Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Laboratoire de Microbiologie Signaux et Microenvironnement (LMSM), Université Abdou Moumouni [Niamey] |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Exudate [SDV.BIO]Life Sciences [q-bio]/Biotechnology Arabinogalactan proteins Plant Science [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Biology Polysaccharide 01 natural sciences root exudates Cell wall 03 medical and health sciences chemistry.chemical_compound Arabinogalactan [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Botany [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] medicine [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Pectobacterium atrosepticum mucilage [SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis Solanum tuberosum 2. Zero hunger chemistry.chemical_classification border cells exudates fungi food and beverages [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular Networks [q-bio.MN] [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology galactose-containing polymers Original Articles root Elicitor [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding 030104 developmental biology [CHIM.POLY]Chemical Sciences/Polymers chemistry Biochemistry Galactose medicine.symptom 010606 plant biology & botany |
| Zdroj: | Annals of Botany Annals of Botany, Oxford University Press (OUP), 2016, 118 (4), pp.797-808. ⟨10.1093/aob/mcw128⟩ |
| ISSN: | 0305-7364 1095-8290 |
| Popis: | International audience; Background and aims Potato (Solanum tuberosum) is an important food crop and is grown worldwide. It is, however, significantly sensitive to a number of soil-borne pathogens that affect roots and tubers, causing considerable economic losses. So far, most research on potato has been dedicated to tubers and hence little attention has been paid to root structure and function.Methods In the present study we characterized root border cells using histochemical staining, immunofluorescence labelling of cell wall polysaccharides epitopes and observation using laser confocal microscopy. The monosaccharide composition of the secreted exudates was determined by gas chromatography of trimethylsilyl methylglycoside derivatives. The effects of root exudates and secreted arabinogalactan proteins on bacterial growth were investigated using in vitro bioassays.Key Results Root exudate from S. tuberosum was highly enriched in galactose-containing molecules including arabinogalactan proteins as major components. Treatment of the root with an elicitor derived from Pectobacterium atrosepticum, a soil-borne pathogen of potato, altered the composition of the exudates and arabinogalactan proteins. We found that the growth of the bacterium in vitro was differentially affected by exudates from elicited and non-elicited roots (i.e. inhibition versus stimulation).Conclusions Taken together, these findings indicate that galactose-containing polymers of potato root exudates play a central role in root–microbe interactions. |
| Databáze: | OpenAIRE |
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