Analyses, extensions and comparison of three experimental schemes for measuring (nJCH+DCH)-couplings at natural abundance
| Autor: | Kyryl Kobzar, Burkhard Luy |
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| Rok vydání: | 2007 |
| Předmět: |
Models
Molecular Coupling Coupling constant Nuclear and High Energy Physics Sequence Spin states Chemistry Research Biophysics Analytical chemistry Reproducibility of Results Pulse sequence Condensed Matter Physics Sensitivity and Specificity Biochemistry Carbon Magnetization Models Chemical Heteronuclear molecule Coherence (signal processing) Computer Simulation Biological system Nuclear Magnetic Resonance Biomolecular Algorithms Hydrogen |
| Zdroj: | Journal of Magnetic Resonance. 186:131-141 |
| ISSN: | 1090-7807 |
| DOI: | 10.1016/j.jmr.2007.02.005 |
| Popis: | Three types of experiments for measuring (n)J(CH) heteronuclear long-range coupling constants are examined and extended with state-of-the-art pulse sequence building-blocks: The use of a HMBC with corresponding reference-HSQC for accurate coupling determination is combined with the constant time technique and the conversion of antiphase magnetization into ZQ/DQ-coherences; CPMG-based LR-CAHSQC and BIRD(r,X)-HSQMBC experiments are examined in detail with respect to their coherence transfer properties; finally, the HSQC-TOCSY-IPAP experiment is introduced, a sequence derived from previously published alpha and beta selective HSQC-TOCSYs using a different spin state selection technique and a recently developed ZQ-suppression method. The experiments are characterized with their advantages and disadvantages and compared using strychnine and menthol as standard molecules. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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