Developmental abnormalities associated with deoxyadenosine methylation in transgenic tobacco
| Autor: | Giandomenico Corrado, Simon Ross, Rik Van Blokland, Peter Meyer, Claire Scollan |
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| Rok vydání: | 1998 |
| Předmět: |
Ribosomal Proteins
Site-Specific DNA-Methyltransferase (Adenine-Specific) Methyltransferase DNA Plant Transgene Gene Dosage Plant Science Biology Gene Expression Regulation Plant Gene expression Tobacco Genetics RNA Messenger RNA-Directed DNA Methylation Gene Ribosomal Protein S6 Deoxyadenosines fungi food and beverages Cell Biology Methylation DNA Methylation Plants Genetically Modified Plants Toxic Phenotype RNA Plant Histone methyltransferase DNA methylation |
| Zdroj: | The Plant journal : for cell and molecular biology. 15(4) |
| ISSN: | 0960-7412 |
| Popis: | Summary As in other higher eukaryotes, DNA methylation in plants is predominantly found at deoxycytosine residues, while deoxyadenosine residues are not methylated at significant levels. 6mdA methylation has been successfully introduced into yeast andDrosophilavia expression of a heterologous methyltransferase, but similar attempts in tobacco had, up until now, proved unsuccessful despite the correct expression of a methyltransferase construct. It was unclear whether this result reflected the failure of heterologous methyltransferases to enter the nucleus, or whether 6mdA methylation, which has been shown to interfere with promoter activity, was toxic for plants. Here we show that 6mdA methylation can be successfully introduced into transgenic tobacco plants via expression of the bacterialdamenzyme. The efficiency of 6mdA methylation was directly proportional to expression levels of thedamconstruct, and methylation of all GATC sites was observed in a highly expressing line. Increasing expression levels of the enzyme in different plants correlated with increasingly abnormal phenotypes affecting leaf pigmentation, apical dominance, and leaf and floral structure. Whilst introduction ofdam-specific methylation does not cause any developmental abnormalities in yeast orDrosophila, our data suggest that methylation of deoxyadenine residues in plants interferes with the expression of genes involved in leaf and floral development. |
| Databáze: | OpenAIRE |
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