Subunits in the membranes of chloroplasts of Phaseolus vulgaris, Pisum sativum, and Aspidistra sp
| Autor: | T. E. Weier, A. Harrison, A. H. P. Engelbrecht, E. B. Risley |
|---|---|
| Rok vydání: | 1965 |
| Předmět: |
chemistry.chemical_classification
Cytoplasm Chloroplasts biology Globular protein Cell Membrane Plastid membrane biology.organism_classification Cytoplasmic Granules Pisum law.invention Chloroplast Cell membrane Crystallography Microscopy Electron Membrane medicine.anatomical_structure chemistry law Plant Cells medicine Anatomy Electron microscope Molecular Biology |
| Zdroj: | Journal of ultrastructure research. 13(1) |
| ISSN: | 0022-5320 |
| Popis: | The partitions and frets of chloroplasts of Aspidistra, Phaseolus vulgaris , and Pisum sativum may be resolved after successful fixation (KMnO 4 glutaraldehyde followed by Dalton's or OsO 4 , acrolein followed by Dalton's or OsO 4 , and by OsO 4 alone) into a series of subunits which are interpreted as globular proteins with associated lipids. The fret membrane, about 75 A wide consists of a single row of subunits. Two rows of subunits form the partitions, which are 135 A wide. They are less than twice the width of the two membranes that form them. The subunits consist of a light core with an average diameter of 37 A surrounded by dark rims about 28 A wide. The arrangement of the subunits in the partitions gives the partitions a superficial appearance of being five-layered membranes formed of three dark components about 40 A wide separated by light leaflets at 19 A in width. The central dark component (40 A) because of its relationship with the outer components of the fret and margin membranes should be twice the width of the two outer dark components, or 80 A. The structure of the plastid membrane thus resolved by electron microscopy is compared with its structure as derived from studies with polarized light, X-rays, chemical information, and electron microscopic studies of whole membranes. The concept of membranes formed of spherical subunits is considered in the light of the unit membrane as it relates to the permeability studies of Danielli and the myelin sheath of nerve cells. |
| Databáze: | OpenAIRE |
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