Unusual direct phycoerythrin labeling of B-cells from a splenic marginal zone lymphoma
| Autor: | A Staal-Viliare, T. Tabary, Véronique Latger-Cannard, J. P. Rault, Jacques H. M. Cohen, Brigitte Reveil, J. Didion, Y. Rio |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Pathology medicine.medical_specialty Histology medicine.drug_class Immunoblotting Monoclonal antibody Immunophenotyping Pathology and Forensic Medicine Flow cytometry Antigen medicine Humans Splenic marginal zone lymphoma B cell Aged Fluorescent Dyes Aged 80 and over B-Lymphocytes Staining and Labeling biology medicine.diagnostic_test Splenic Neoplasms Phycoerythrin Lymphoma B-Cell Marginal Zone Cell Biology Flow Cytometry medicine.disease Molecular biology medicine.anatomical_structure Polyclonal antibodies biology.protein |
| Zdroj: | Cytometry Part B: Clinical Cytometry. :189-193 |
| ISSN: | 1552-4957 1552-4949 |
| DOI: | 10.1002/cyto.b.20392 |
| Popis: | Flow cytometry is the most widely used method for lymphocyte subset characterization. Two types of antibodies, directly labeled with fluorochrome, are currently used for immunological diagnosis of B-cell lymphoproliferation: monoclonal antibodies against leukocyte differentiation antigens and polyclonal antibodies against immunoglobulins and light chains. In this study is described the case of a patient with an uncommon immunophenotyping of a B-cell lymphoproliferative disorder. B-cells from peripheral blood and from bone marrow reacted positively with all the tested phycoerythrin (PE)-conjugated antibodies, including the isotypic control. So we thought about a B-cell proliferation carrying a surface receptor recognizing PE: these B-cells were directly labeled with streptavidin-PE, indeed. Moreover, the immunodots from the patient were able to fix the streptavidin-PE. Finally, this unusual immunophenotyping was solved by using antibodies labeled with other fluorochromes than PE. q 2007 Clinical Cytometry Society |
| Databáze: | OpenAIRE |
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