Influence of Ionic Strength of Incubation Medium on the Release of Some Enzymes from Rat Liver Mitochondria
| Autor: | A. M. Gerasimov, Leonid F. Panchenko, Loktaeva Td |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Adenylate kinase
Digitonin Mitochondria Liver Mitochondrion Cell Fractionation Biochemistry Potassium Chloride Sonication Glutamate Dehydrogenase Centrifugation Density Gradient Animals Inner membrane Carbon Tetrachloride Incubation chemistry.chemical_classification Glutamate dehydrogenase Adenylate Kinase Osmolar Concentration Phosphotransferases Isocitrate Dehydrogenase Rats Isocitrate dehydrogenase Enzyme chemistry Ionic strength |
| Zdroj: | Enzyme. 19:337-347 |
| ISSN: | 2504-2564 0013-9432 |
| DOI: | 10.1159/000459008 |
| Popis: | Influence of ionic strength of incubation medium on the release of adenylate kinase (AK), isocitrate dehydrogenase (IDH) and glutamate dehydrogenase (GDH) from intact and injured rat liver mitochondria has been studied. It has been found that raised ionic strength (mu equal 0.16-0.32) has increased the release of GDH and AK activities - IDH to a lesser degree - for mitochondrial preparations only with damaged inner membrane. Treatment of mitochondria with potassium chloride solution of definite ionic strength has permitted to detect the alteration of these organelles occurring in vivo and in vitro. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|