Homeotic Function of Drosophila Bithorax-Complex miRNAs Mediates Fertility by Restricting Multiple Hox Genes and TALE Cofactors in the CNS
| Autor: | Ernesto Sánchez-Herrero, Fernando Bejarano, Eric C. Lai, Daniel L. Garaulet, Douglas W. Allan, David M. Tyler, Piero Sanfilippo, Monica C. Castellanos |
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| Rok vydání: | 2014 |
| Předmět: |
Central Nervous System
animal structures Molecular Sequence Data Oviducts Biology Article General Biochemistry Genetics and Molecular Biology Immunoenzyme Techniques Sexual Behavior Animal 03 medical and health sciences 0302 clinical medicine Sequence Homology Nucleic Acid Image Processing Computer-Assisted Animals Drosophila Proteins Hox gene Molecular Biology Transcription factor In Situ Hybridization Body Patterning 030304 developmental biology Homeodomain Proteins Motor Neurons Regulation of gene expression Genetics 0303 health sciences Base Sequence Genes Homeobox Gene Expression Regulation Developmental Cell Biology biology.organism_classification MicroRNAs Drosophila melanogaster Larva Multigene Family Bithorax complex Homeobox Female Homeotic gene 030217 neurology & neurosurgery Drosophila Protein Transcription Factors Developmental Biology |
| Zdroj: | Developmental Cell. 29:635-648 |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2014.04.023 |
| Popis: | Summary The Drosophila Bithorax complex (BX-C) Hox cluster contains a bidirectionally transcribed miRNA locus, and a deletion mutant (Δ mir ) lays no eggs and is completely sterile. We show these miRNAs are expressed and active in distinct spatial registers along the anterior-posterior axis in the CNS. Δ mir larvae derepress a network of direct homeobox gene targets in the posterior ventral nerve cord (VNC), including BX-C genes and their TALE cofactors. These are phenotypically critical targets, because sterility of Δ mir mutants was substantially rescued by heterozygosity of these genes. The posterior VNC contains Ilp7+ oviduct motoneurons, whose innervation and morphology are defective in Δ mir females, and substantially rescued by heterozygosity of Δ mir targets, especially within the BX-C. Collectively, we reveal (1) critical roles for Hox miRNAs that determine segment-specific expression of homeotic genes, which are not masked by transcriptional regulation; and (2) that BX-C miRNAs are essential for neural patterning and reproductive behavior. |
| Databáze: | OpenAIRE |
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