The Cdk5 inhibitor roscovitine strongly inhibits glucose uptake in 3T3-L1 adipocytes without altering GLUT4 translocation from internal pools to the cell surface
| Autor: | Ignacio V. Sandoval, Gemma Muruais, Vassiliki Lalioti |
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| Rok vydání: | 2009 |
| Předmět: |
Snf3
Time Factors Physiology Glucose uptake Clinical Biochemistry Cell Chromosomal translocation Deoxyglucose Mice 3T3-L1 Cells Adipocytes Roscovitine medicine Animals Insulin Protein Kinase Inhibitors Glucose Transporter Type 4 Dose-Response Relationship Drug biology Cyclin-dependent kinase 5 Cell Membrane Glucose transporter Biological Transport Cyclin-Dependent Kinase 5 3T3-L1 Cell Biology Cell biology Protein Transport medicine.anatomical_structure Biochemistry Purines biology.protein GLUT4 |
| Zdroj: | Journal of Cellular Physiology. 220:238-244 |
| ISSN: | 1097-4652 0021-9541 |
| DOI: | 10.1002/jcp.21758 |
| Popis: | Glucose entry into mammalian cells is facilitated by a family of glucose transport proteins known as GLUTs. Treatment of 3T3-L1 adipocytes with the Cdk5 inhibitor roscovitine strongly inhibits insulin-stimulated/GLUT4-mediated glucose transport. Inhibition of glucose uptake occurs within 2–6 min of the addition of roscovitine and is slowly reversed. The roscovitine treatment interferes with neither the translocation nor the insertion of GLUT4 into the plasma membrane. These studies support recent evidence showing that insulin-stimulated Cdk5 is implicated in the regulation of GLUT4-mediated glucose uptake in 3T3-L1 adipocytes. J. Cell. Physiol. 220: 238–244, 2009. © 2009 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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