Immunological basis for protection in a murine model of tick-borne encephalitis by a recombinant adenovirus carrying the gene encoding the NS1 non-structural protein
| Autor: | Galina G. Karganova, L B Elbert, S. V. Ozherelkov, A V Pronin, A V Timofeev, Deeva Av, J. R. Stephenson |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Genes Viral viruses Viral Nonstructural Proteins Antibodies Viral Virus law.invention Adenoviridae Encephalitis Viruses Tick-Borne Mice Immune system law Virology medicine Animals Gene Recombination Genetic Immunity Cellular Mice Inbred BALB C biology Tick-borne encephalitis Defective Viruses Viral Vaccines biochemical phenomena metabolism and nutrition medicine.disease Acquired immune system Adoptive Transfer Disease Models Animal Recombinant DNA biology.protein Cytokines Female Antibody Encephalitis Encephalitis Tick-Borne |
| Zdroj: | The Journal of general virology. 79 |
| ISSN: | 0022-1317 |
| Popis: | The humoral immune response to flaviviruses is mainly directed to the major envelope protein, E, and a glycosylated non-structural protein, NS1. Cell-mediated immune responses, however, appear to be directed mainly against non-structural proteins. Experiments described here show that a defective recombinant adenovirus (Rad51) containing the gene encoding the NS1 protein of tick-borne encephalitis virus can induce a strong protective immune response against several pathogenic tick-borne flaviviruses in an experimental animal model, and can enhance the efficacy of conventional vaccine preparations. A protective immune response against a lethal virus challenge can also be induced by the passive transfer of antibodies, B cells or T cells from animals vaccinated with Rad51. Raised levels of non-neutralizing antibodies and cytokines associated with a T helper cell-type 1 immune response are also observed. These data demonstrate the importance of non-structural viral proteins in the protective immune response against flaviviruses and support the use of non-structural viral proteins as vaccine components. |
| Databáze: | OpenAIRE |
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