A New Method of Urea Removal Using Urease and Expanded Polytetrafluoroethylene Membrane
| Autor: | Kazuo Era, Shuji Suwa, Tetsuzo Agishi, Iwakazu Kaneko, Kazuo Ota, Kazuo Aida |
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| Rok vydání: | 2008 |
| Předmět: |
Urease
Inorganic chemistry Biomedical Engineering Medicine (miscellaneous) Bioengineering In Vitro Techniques Biomaterials chemistry.chemical_compound Ammonia Dogs Renal Dialysis Animals Urea Charcoal Polytetrafluoroethylene Chromatography biology Chemistry Extraction (chemistry) Membranes Artificial General Medicine Permeation Enzymes Immobilized Solutions Membrane visual_art visual_art.visual_art_medium biology.protein Hydrochloric Acid Dialysis (biochemistry) |
| Zdroj: | Artificial Organs. 3:350-353 |
| ISSN: | 1525-1594 0160-564X |
| DOI: | 10.1111/j.1525-1594.1979.tb01076.x |
| Popis: | A new method for urea removal using a gas membrane is introduced along with some preliminary results. The membrane used was expanded polytetrafluoroethylene (E-PTFE) which is highly permeable to gaseous substances, while at the same time it is highly resistant to water permeation. In in vitro experiments using 10 mmol/L ammonia solution it was revealed that the single-pass reduction rate was approximately 95% at 30°C at a flow rate of 200 ml/min. In animal experiments using four dogs, the extraction rate of urea was 40.4 ± 4.4% after four hours of dialysis using 5 L dialysate. However, elevation of blood ammonia was observed in all dogs tested. Removal of ammonia by means of a gas membrane is considered to be feasible and has the possibility of being used for maintenance hemodialysis in combination with urease and charcoal. |
| Databáze: | OpenAIRE |
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