Chitosan-Gelatin Scaffolds Incorporating Decellularized Platelet-Rich Fibrin Promote Bone Regeneration
| Autor: | Xiaoyan Wang, Xiangnan Song, Anlong Jiang, Jinglong Yan, Guanghua Chen, Wei Zhao, Hui Chi, Tailong Yu, Chengchao Song, Leyu Zheng |
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| Rok vydání: | 2021 |
| Předmět: |
Scaffold
food.ingredient Decellularization Biocompatibility 0206 medical engineering Biomedical Engineering 02 engineering and technology Bone healing 021001 nanoscience & nanotechnology 020601 biomedical engineering Gelatin Platelet-rich fibrin Biomaterials Chitosan chemistry.chemical_compound food chemistry 0210 nano-technology Bone regeneration Biomedical engineering |
| Zdroj: | ACS biomaterials scienceengineering. 5(10) |
| ISSN: | 2373-9878 |
| Popis: | Platelet-rich fibrin (PRF), which functions as a growth factor carrier, has been extensively used to promote soft and hard tissue repair. However, whether decellularized PRF (DPRF) maintains its bioactive effects is unknown. Chitosan/gelatin(C/G) base scaffolds display appropriate biocompatibility and mechanical properties, but they lack biological activity. Thus, the incorporation of DPRF into the C/G scaffold can theoretically improve both the bioactivity of the C/G scaffold and the strength of PRF. In this study, DPRF was prepared using a method combining repeated freeze-thawing and enzymatic digestion. Also, DPRF-loaded chitosan-gelatin scaffolds (C/G/DPRF) were fabricated, using C/G scaffolds as controls. The osteogenic potential of scaffolds was investigated in vitro and in vivo. Compared with the C/G scaffold, C/G/DPRF had a larger pore size (280.8 ± 11.7 μm vs 235.0 ± 11.6 μm; P 0.05) but reduced compressive modulus (0.81 ± 0.02 MPa vs 1.17 ± 0.05 MPa; P < 0.05). In vitro, C/G/DPRF scaffolds accelerated attachment, proliferation, and osteogenesis-related marker expression of bone marrow stem cells. In vivo, C/G/DPRF scaffolds led to enhanced bone healing and defect closure in a rat calvarial defect model. Thus, we concluded that DPRF remains bioactive and the prepared C/G/DPRF scaffold is a promising material for bone regeneration. |
| Databáze: | OpenAIRE |
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