Peroxisome proliferator activated receptor-gamma stimulation for prevention of 5-fluorouracil-induced oral mucositis in mice
| Autor: | Mariangela Sottili, Isacco Desideri, Chiara Gerini, Roberta Grassi, Giulia Salvatore, M. Perna, Icro Meattini, Giampaolo Biti, Giulio Francolini, Francesca Castiglione, Mauro Loi, Daniela Greto, Pierluigi Bonomo, Lorenzo Livi, Monica Mangoni |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Blotting Western Peroxisome proliferator-activated receptor Pharmacology Matrix metalloproteinase Real-Time Polymerase Chain Reaction Rosiglitazone 03 medical and health sciences Mice 0302 clinical medicine medicine Mucositis Animals Oral mucosa chemistry.chemical_classification Chemotherapy Stomatitis business.industry Mouth Mucosa Interleukin medicine.disease Mice Inbred C57BL PPAR gamma 030104 developmental biology medicine.anatomical_structure Otorhinolaryngology chemistry 030220 oncology & carcinogenesis Cytokines Fluorouracil business Infiltration (medical) medicine.drug |
| Zdroj: | Headneck. 40(3) |
| ISSN: | 1097-0347 |
| Popis: | Background Oral mucositis is a side effect of treatment regimens containing 5-fluorouracil (5-FU). The purpose of this study was to present our evaluation to see if rosiglitazone (RGZ) protected normal tissues from chemotherapy-induced oral mucositis. Methods C57BL/6J mice were treated with 5-FU for 5 days, with or without RGZ. Mice were euthanized after 5, 8, 11, or 15 days, and mucosal segments were collected. Results The RGZ did not affect the 5-FU-induced decrease in mouse body weight. The 5-FU caused loss of epithelial architecture, collagen fiber impairment, and inflammatory infiltration. The RGZ reduced leukocyte infiltration, preserved tissue structure, and dampened the 5-FU-induced expression of p53 and matrix metalloproteinase (Mmp)-2 after 5 days, and of Mmp-2 and interleukin (Il-1β after 15 days. The RGZ inhibited the 5-FU-induced increase of transforming growth factor-beta (TGF-β) and nuclear factor-kappa B (NF-κB) proteins and restored collagen protein levels. Conclusion The RGZ had a protective effect on oral mucosa damaged by chemotherapy. These data encourage the further study of RGZ for the prevention of 5-FU-induced mucositis in patients with cancer. |
| Databáze: | OpenAIRE |
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