TaSnRK2.4 is a vital regulator in control of thousand-kernel weight and response to abiotic stress in wheat
| Autor: | Hong-juan Zhang, Xiulin Liu, Rui-lian Jing, Hong-ji Zhang, Yu-ying Li, Lili Miao, Xiao-ping Chang, Jingyi Wang, Xin-guo Mao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
abiotic stress Agriculture (General) Regulator Plant Science 01 natural sciences Biochemistry S1-972 Food Animals TaSnRK2.4 Arabidopsis Luciferase Allele Protein kinase A Genetics Abiotic component Ecology biology Kinase Abiotic stress 04 agricultural and veterinary sciences biology.organism_classification association analysis TKW 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Animal Science and Zoology Agronomy and Crop Science 010606 plant biology & botany Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 20, Iss 1, Pp 46-54 (2021) |
| ISSN: | 2095-3119 |
| Popis: | Sucrose non-fermenting 1-related protein kinase 2 (SnRK2) is a plant-specific serine/threonine kinase involved in response to adverse environmental stimuli. Previous studies showed that TaSnRK2.4 was involved in response to abiotic stresses and conferred enhanced tolerance to multiple stresses in Arabidopsis. Further experiments were performed to decipher the underlying mechanisms and discover new functions. The genomic sequences of TaSnRK2.4s locating on chromosome 3A, 3B and 3D were obtained. Sequencing identified one and 13 variations of TaSnRK2.4–3A and TaSnRK2.4-3B, respectively, but no variation was detected in TaSnRK2.4-3D. The markers 2.4AM1, 2.4BM1 and 2.4BM2 were developed based on three variations. Association analysis showed that both TaSnRK2.4-3A and TaSnRK2.4-3B were significantly associated with thousand-kernel weight (TKW), and that SNP3A-T and SNP3B-C were favorable alleles for higher TKW. Yeast two-hybrid and split luciferase assays showed that TaSnRK2.4 physically interacted with abiotic stress responsive protein TaLTP3, suggesting that TaSnRK2.4 enhanced abiotic stress tolerance by activating TaLTP3. Our studies suggested that TaSnRK2.4 have potential in improving TKW and response to abiotic stress. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect