Influence of the reporter vector backbone on 2-deoxyglucose dependent promoter activation
| Autor: | Mateusz Wawro, Aleksandra Wielento, Joanna Jurczak, Aleksandra Solecka, Weronika Sowinska, Aneta Kasza |
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| Rok vydání: | 2020 |
| Předmět: |
Genetic Vectors
Gene Expression Matrix (biology) Astrocytoma Deoxyglucose Transfection General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Cell Movement Genes Reporter Luciferases Firefly Cell Line Tumor Gene expression Humans Glycolysis metalloproteinase 2 Vector (molecular biology) Promoter Regions Genetic Gene 0303 health sciences Chemistry 030302 biochemistry & molecular biology Cell biology 2-deoxyglucose Cell culture gene expression Matrix Metalloproteinase 2 reporter vector Plasmids |
| Zdroj: | Acta biochimica Polonica. 67(3) |
| ISSN: | 1734-154X |
| Popis: | Reporter vectors are very often used to investigate mechanisms responsible for regulation of promoter activity. Since their first generation, many new variants were constructed to increase sensitivity and reduce background signal. However, these tools are still imperfect and can generate false results. We have found that depending on the backbone of the reporter vector, pGL3 or pGL2, different results are obtained for a eukaryotic promoter’s activation by metabolic changes. These observations were done in the course of investigation of the MMP2 (matrix metalloproteinase-2) promoter regulation in response to inhibition of glycolysis. |
| Databáze: | OpenAIRE |
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