Imaging of growth factors on a human tooth root canal by surface-enhanced Raman spectroscopy
| Autor: | Zuzana Chaloupková, Juraj Ševčík, Václav Ranc, Radek Zbořil, Radovan Žižka |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Silver Materials science Root canal Bone Morphogenetic Protein 2 Spectrum Analysis Raman Biochemistry Nanocomposites Analytical Chemistry 03 medical and health sciences symbols.namesake 0302 clinical medicine Tissue engineering Transforming Growth Factor beta Human tooth medicine Fluorescence microscope Humans Nanocomposite 030206 dentistry Surface-enhanced Raman spectroscopy Ferrosoferric Oxide 030104 developmental biology medicine.anatomical_structure Dentin symbols Pulp (tooth) Fibroblast Growth Factor 2 Dental Pulp Cavity Raman spectroscopy Biomedical engineering |
| Zdroj: | Analytical and Bioanalytical Chemistry |
| ISSN: | 1618-2650 1618-2642 |
| Popis: | Endodontic treatment of immature permanent teeth with necrotic pulp poses several clinical challenges and is one of the most demanding interventions in endodontics. Recently, with new discoveries in the field of tissue engineering, novel treatment protocols have been established. The most promising treatment modality is revascularization, whose integral part is the exposure of collagen matrix and embedded growth factors. However, optimization of the treatment protocol requires a development of analytical procedures able to analyze growth factors directly on the sample surface. In this work, method based on surface-enhanced Raman spectroscopy (SERS) was developed to investigate the influence of the time of the medical treatment using EDTA on exposure and accessibility of the growth factors, namely TGF-s1, BMP-2, and bFGF on the dentine surface. The nanotags, which consist of magnetic Fe3O4@Ag nanocomposite covalently functionalized by tagged antibodies (anti-TGF-s1-Cy3, anti-BMP-2-Cy5, and anti-bFGF-Cy7), were employed as a SERS substrate. Each antibody was coupled with a unique label allowing us to perform a parallel analysis of all three growth factors within one analytical run. Developed methodology presents an interesting alternative to a fluorescence microscopy and in contrary allows evaluating a chemical composition and thus minimizing possible false-positive results. Graphical abstract. |
| Databáze: | OpenAIRE |
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