Organized Aggregation of Porphyrins in Lipid Bilayers for Third Harmonic Generation Microscopy

Autor: Liyang Cui, Juan Chen, Richard Cisek, Gang Zheng, Danielle Tokarz, Virginijus Barzda, Kenneth K. Ng, Fan Wang
Rok vydání: 2015
Předmět:
Zdroj: Angewandte Chemie. 127:14134-14138
ISSN: 1521-3757
0044-8249
DOI: 10.1002/ange.201506171
Popis: Nonlinear optical microscopy has become a powerful tool for high-resolution imaging of cellular and subcellular composition, morphology, and interactions because of its high spatial resolution, deep penetration, and low photo-damage to tissue. Developing specific harmonic probes is essential for exploiting nonlinear microscopic imaging for biomedical applications. We report an organized aggregate of porphyrins (OAP) that formed within lipidic nanoparticles showing fingerprint spectroscopic properties, structure-associated second harmonic generation, and superradiant third harmonic generation. The OAP facilitated harmonic microscopic imaging of living cells with significantly enhanced contrast. The structure-dependent switch between harmonic (OAP-intact) and fluorescence (OAP-disrupted) generation enabled real-time multi-modality imaging of the cellular fate of nanoparticles. Robustly produced under various conditions and easily incorporated into pre-formed lipid nanovesicles, OAP provides a biocompatible nanoplatform for harmonic imaging.
Databáze: OpenAIRE
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