Comparison of arbitrarily primed polymerase chain reaction, ribotyping, and monoclonal antibody analysis for subtyping Legionella pneumophila serogroup 1
| Autor: | R F Benson, W T Martin, P Gomez-Lus, S P O'Connor, C M Black, B S Fields |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Microbiology (medical)
Serotype DNA Bacterial medicine.drug_class Molecular Sequence Data Monoclonal antibody Legionella pneumophila Microbiology law.invention Disease Outbreaks Ribotyping law medicine Humans Legionella pneumophila Serogroup 1 Serotyping Polymerase chain reaction biology Base Sequence Antibodies Monoclonal DNA Restriction Enzymes Amplicon biology.organism_classification bacterial infections and mycoses Virology Subtyping Bacterial Typing Techniques Evaluation Studies as Topic Legionnaires' Disease DNA Probes Research Article |
| Zdroj: | Journal of clinical microbiology. 31(7) |
| ISSN: | 0095-1137 |
| Popis: | Arbitrarily primed polymerase chain reaction (AP-PCR) was used to characterize Legionella pneumophila serogroup 1. Cells from a single colony could be subtyped by AP-PCR within a few hours. The discrimination between strains of L. pneumophila serogroup 1 by AP-PCR was equivalent to that by monoclonal antibody analysis and ribotyping. Four strains representing the monoclonal antibody pattern most frequently associated with outbreaks all yielded unique amplicon patterns by AP-PCR. |
| Databáze: | OpenAIRE |
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